Standard Protocol for Inducing Atopic Dermatitis by "Biosta AD"
This is an introduction to the standard protocol for inducing atopic dermatitis using BioSta AD.
In the atopic dermatitis model using Biosta AD, after destroying the skin barrier with 4% SDS, the product is applied to the back and ear area of the mice at a dose of 100 mg/mouse twice a week. The induction period is approximately 3 weeks (for a total of 6 applications). Ultimately, a total of 600 mg of the product will be applied. In this model, symptoms begin to appear from 1 to 2 weeks after the start of application, with an incidence rate of about 80% observed at 3 to 4 weeks. 【Initial Induction Method】 ○(1) NC/Nga mice (female, from Charles River Japan, aged 10 weeks or older) are anesthetized (with ether), and the back and ear areas are shaved with clippers, followed by the application of an appropriate amount of depilatory agent to remove hair. ○(2) After wiping off the depilatory agent, 100 mg of Biosta AD is evenly applied to the back and ear areas. ○(3) After application, the mice are returned to their breeding cages. For more details, please contact us or download the catalog.
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